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KMID : 0043320120350081421
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Archives of Pharmacal Research
2012 Volume.35 No. 8 p.1421 ~ p.1430
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Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells
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Yoon Weon-Jong
Heo Soo-Jin
Han Sang-Chul
Lee Hye-Ja
Kang Gyeoung-Jin
Kang Hee-Kyoung
Hyun Jin-Won
Koh Young-Sang
Yoo Eun-Sook
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Abstract
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A study on the anti-inflammatory activity of brown alga Sargassum siliquastrum led to the isolation of sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dosedependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS-induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation.
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KEYWORD
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Sargachromanol G, Sargassum siliquastrum, Inflammatory markers, Pro-inflammatory cytokines, NF-κB, MAPK
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